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Table 1 Characteristics of 24 polymorphic microsatellite loci in 57 specimens of sheepshead (Archosargus probatocephalus) from the Indian River, Florida

From: Isolation and characterization of 24 polymorphic microsatellite loci for the study of genetic population structure of the sheepshead Archosargus probatocephalus (Actinopterygii, Perciformes, Sparidae)

Locus

Primer sequence (5′→3′) forward/reverse

Repeat motif

Allele size range

Ka

H O

H E

PIC

GenBank accession no.

Apro01

CACAATCACAAACAATACAAACACAT

ACTAATGCCTTTCTGTCTGCA

(AC)7/(AC)24

119–205

20

0.66

0.85

0.84

KU516013

Apro02

AAAGCGGTTCAGAAGGTTTATTTGT

GACAGCACAGAACAGGTTGATATAG

(GT)17

100–132

16

0.89

0.89

0.87

KU516014

Apro03

TATAACGATAACACTGCAGAAAGAGC

CCCTTTTAAATACGACTAGGACAAAC

(AC)33

168–230

24

0.82

0.90

0.88

KU516015

Apro04

GAAATTTCCTTTAACCTTTTCTCTCA

GACCAGCTGTTTATAGTTTCAACAAA

(AC)7/(AC)8

165–205

16

0.83

0.91

0.89

KU516016

Apro05

GAGATTGTTTTGTGCCATCTCTGTT

ATATTATTCAGACACACGGGTGGATT

(GT)22

149–203

21

0.91

0.90

0.88

KU516017

Apro06

CTAGTCAAAACACCTTTAACCACCTT

CCATGTTCGTCTTGAAACACTTTT

(GT)4/(GT)7

202–224

9

0.85

0.80

0.77

KU516018

Apro10

GCTGATGGTACAGATGACGATAGAG

GTAAAACCTTGGAGGTCTCACTCTC

(GT)6/(GT)5/ (GT)10

152–222

17

0.75

0.82

0.79

KU516019

Apro12

TCTACTATCAGCCAAGAGTAAAGCA

AGACGTATGTGCATATGTATGGGTA

(CA)11

146–162

5

0.70

0.61

0.53

KU516020

Apro13

CTTGACTTCACCTTCACTTCACCTT

ATGAACCGAGAAACAACATTACAGT

(AC)15/(AC)15

152–164

6

0.66

0.70

0.64

KU516021

Apro17

GTATGACTCCAAACCCTCCAGTC

CAGCCTTGTGTACTGTTGTTTGTAG

(GT)7/(TG)5

118–190

21

0.91

0.91

0.89

KU516022

Apro18

AATAAAAGGTGCTGCATGTAGTCATA

ATGATACTCCAATCAGTCCACAGTC

(CA)7(TA)4

167–171

3

0.31

0.29

0.26

KU516023

Apro19

TAACAATGGATTACATCAGTCATGC

TTATCCAGCTTAGACTCCCACATAC

(GT)27

126–180

23

0.97

0.95

0.94

KU516024

Apro21

CACAGGGATGAGAGTATACAGTACG

TTCTTCAAAAAGGCTGTTCTCTTTA

(GT)9/(TG)7 (CT)2(GT)6

139–177

10

0.64

0.80

0.76

KU516025

Apro22

AGCTTTGACAGACACTGAGTAACAAC

GGGTTCTGATGGATATAGAGTAGCAG

(AC)16

141–157

9

0.78

0.80

0.76

KU516026

Apro25

TTCTAAAAATGATGTGTGTATGTCTGT

ACCTGGGCCAGAGGATACTACTACT

(TG)3/(TG)8

122–184

10

0.76

0.81

0.78

KU516027

Apro27

AACAGATTAATGGAATCTCCTTCTGT

AAGCACAGCTATTGTTTATGTACGAG

(AC)8

163–167

3

0.14

0.14

0.13

KU516028

Apro28

TCTCATACAGTATTTGTCCCTCCTC

ATTGCTTAGCATTTAACGAGAAAGA

(TC)7TTT(TC)10

105–153

9

0.42

0.37

0.35

KU516029

Apro29

GCCTAGCATCATTCTGTCACTCAC

ATTCGATGAATTCAGTATGAGTTTGA

(CA)14

162–196

11

0.65

0.70

0.67

KU516030

Apro30

CATCCAATTGTGAGAGGTGTCAG

ACACGCTAATTGGAGTTTGATGA

(CA)9

123–161

17

0.85

0.88

0.87

KU516031

Apro32

CAGAGCTGATATTGCCAGATGAAT

TAGTTAACAGTTTGGTGGATTTTCC

(RC)17

119–129

4

0.09

0.34*

0.30

KU516032

Apro35

TAAAGAATTCATGGGATAAAGCTCA

TTTGTCAGTCTGCATCATGAAGTTA

(CA)12

173–219

17

0.86

0.89

0.87

KU516033

Apro38

CTGTGCAGGTCTGTTTGTCTCC

CATTAATTCATTGTTAACGGCAAA

(TG)3 TC(TG)5

117–119

2

0.30

0.49

0.37

KU516034

Apro39

AGTTCCTCTCGTCTCCTTTTCAAG

AGAGTGTGTCGTTTTGCATTTCTT

(AC)14

126–176

15

0.81

0.84

0.82

KU516035

Apro40

TCAAAATCAATTTAGCAAAATAGGAAA

AACGGCTGTAACATTATGTGTGTTT

(G)13(A)5 (C)14/(A)10

144–206

27

0.78

0.95*

0.94

KU516036

 

Mean

  

13.1

0.68

0.73

0.70

 
  1. Ka number of alleles, H O observed heterozygosity, H E expected heterozygosity, PIC polymorphic information content
  2. * Indicates significant departure from HWE
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BMC Research Notes

ISSN: 1756-0500

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