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Table 5 Relative assembly scores for different read lengths

From: Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly

Quality metric

Read length

Genome

Bce

Efa

Sen

Pst

Corrected NG50

100

0.52

0.68

0.54

0.996

125

0.82

0.90

0.79

0.995

150

0.90

0.96

0.81

1

175

1

1

0.95

0.995

189

0.91

0.996

1

0.99

max**

541,348

419,532

358,280

213,754

NGA50

100

0.55

0.95

0.71

1

125

0.75

1

0.86

0.86

150

0.78

0.97

0.85

0.96

175

0.996

0.99

0.94

0.98

189

1

0.999

1

0.97

max**

898,406

424,840

455,293

249,244

  1. Read length associated relative assembly scores are listed per genome for SPAdes assemblies. Relative assembly scores for Sen were determined in dependence on 3 assembly sets each. Each other genome comprised 6 assembly sets per relative assembly score
  2. max** Absolute value in nucleotides of the maximal relative assembly score of the column
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BMC Research Notes

ISSN: 1756-0500

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