Fig. 5
Standard curves for the quantification of SAM (a) and SAH (b) by the direct cELISA. The x-axis shows the values of the base 10 logarithm of the concentrations of the standards. The y-axis is expressed as the LOGIT, which is defined as Ln(A/AS0/(1 − A/AS0)), where A is the OD450 value of the standard and AS0 is the OD450 value of the control (without antigen). a HRP-118-6 was diluted 1:22,000, 1:25,000, 1:30,000 and 1:32,000. The standard curve equations for the 3 different diluted tests are shown in the figure. b Different lots of HRP-301-1 and different dilutions were used. The standard curve equations for the 3 tests are shown