Fig. 4
CspA protein production and growth of Staphylococcus aureus Newman. a The Western blot of CspA production by the wild type S. aureus Newman is the upper band. The lower band is the Western blot of a negative control S. aureus cspA mutant SKN23. Lane 1 contains 35 ng of purified His-tag CspA control. In both the wild type and mutant Western blots, Lanes 2, 3, and 4 contain 30 µg of total soluble protein from aliquots of cells taken at 1, 3, and 6 h. The Lane, Control, and h for each sample are labeled. b The relative expression values for CspA bands were calculated using Image Lab 5.2.1 software following a total protein normalization method. The relative expression of CspA for each time point, 1, 3, and 6 h, is represented by black columns with double-ended bars for the standard error of the mean (SEM) (left Y axis). The OD600 for each sample is represented by a gray circle and time points connected by blue lines with doubled-ended error bars for the SEM (right Y axis). The double-ended error bars are not shown if the SEM is less than the width of symbol. The OD600 sample for 6 h was diluted 1:2 before measurement