Differential expression of the inflammatory ciita gene may be accompanied by altered bone properties in intact sex steroid-deficient female rats

Table 1 Bone biomarker levels in rat strains differentially expressing the Ciita gene

	DA-sham (n = 4)	DA-OVX (n = 7–8)	VRA4-sham (n = 2)	VRA4-OVX (n = 9–10)	Comparison - overall effects of strain or OVX			(%) Change following OVX
					(i) Strain	(ii) OVX	(iii) Interaction	(iv) DA	(v) VRA4
Week 3
U-CTX-1	25.5 ± 13.1	32.5 ± 19.4	17.5 ± 8.9	34.0 ± 18.3	ns	ns	ns	NA	NA
U-OC	21.1 ± 10.4	55.6 ± 29.7 (7^a)	37.1 ± 12.9	100.0 ± 40.5 ^b *#	ns	p < 0.01	ns	163 ± 141	169 ± 109
*Week 16*
U-CTX-1	8.5 ± 3.5	9.6 ± 2.4 (7^d)	9.6 ± 2.5	12.8 ± 3.2 (9^d)	ns	ns	ns	NA	NA
U-OC	9.1 ± 5.4	13.6 ± 5.6 (7^d)	8.0 ± 1.5	20.6 ± 9.4 (9,^c,d)	ns	p < 0.05	ns	48 ± 61	157 ± 117^*
S-CTX-1	18.2 ± 2.6	20.3 ± 7.0	17.4 ± 1.9	19.0 ± 3.4	ns	ns	ns	NA	NA
S-P1NP	10.2 ± 1.4	8.1 ± 1.5	9.2 ± 1.3	8.3 ± 2.4	ns	ns	ns	NA	NA
S-OC	172.8 ± 24.1	198.1 ± 17.0	283.7 ± 55.6 ^**	218.5 ± 52.2	NA	NA	p < 0.05	145 ± 10	-23 ± 18^***

Biomarker values are mean ± SD. Urinary (mg/mmol), serum (ng/ml). Urine CTX-1 and osteocalcin are creatinine corrected. NA, not applicable. Ns, not significant
^aInsufficient sample volume for quantification in one animal. Single-measurement for ^bone or ^cthree samples (insufficient volume for double-measurements). ^dInsufficient sample volume to quantify creatinine in one animal
For U-OC, values < LLOQ were set to nominal LLOQ (57.3 ng/ml) for: Week 3, 1x sample each in DA-sham, DA-OVX, and VRA4-OVX; Week 16, all samples in DA-sham, 1x sample each in VRA4-sham and DA-OVX, and 3x samples in VRA4-OVX.
For U-OC, values > ULOQ were set to nominal ULOQ (959 ng/ml) for: Week 3, 1x sample each in VRA4-sham and VRA4-OVX groups
For comparisons with corresponding DA-group *P < 0.05, **P < 0.01. For comparisons with VRA4-sham ^# P < 0.05
See also Supplementary Figs. 1–4

ISSN: 1756-0500