Table 1 Tetra-primer ARMS-PCR primers for the detection of OPRM1 gene rs1799971 variant
Primers | Sequence (5’ to 3’) | Length (bp) | Tm (manufacturer) (oC) | Product Size (bp) |
|---|---|---|---|---|
Forward inner primer (G allele) | GGGTCAACTTGTCCCACTTAGATGTCG | 27 | 66.2 | Control: 395 (G): 186 (A): 257 |
Reverse inner primer (A allele) | ACCGCATGGGTCGGACAGTTT | 21 | 66.6 | |
Forward outer primer (5’ − 3’) | GTAAGAAACAGCAGGAGCTGTGGCAG | 26 | 65.7 | |
Reverse outer primer (5’ − 3’) | CATGACCAGGAAGTTTCCGAAGAGC | 25 | 65.0 |