Fig. 1
From: Genome sequence of a virulent and hypermucoviscous-like Klebsiella michiganensis clinical isolate
Assays for determining the capsule-associated virulence phenotype in K. michiganensis 9273. A Quantification of uronic acid and B Phagocytosis assay. We included one hypervirulent K. pneumoniae (14660, ST86-K2) and one classical and non-hypermucoviscous K. pneumoniae (9468) as reference strains for capsule production and phagocytosis resistance assays. C Kaplan–Meier survival curves of infected G. mellonella larvae with K. michiganensis isolate 9273 at three doses 1 × 104, 1 × 105 and 1 × 106 CFUs. K. variicola F2R9 (106 CFUs) [12], K. pneumoniae 9468 (106 CFUs) and hypervirulent K. pneumoniae 14660 (104 CFUs) were used respectively as nonlethal and lethal doses control. Statistical analysis was carried out based on one way ANOVA (A and B), long rank (Mantel-Cox) and Chi-square test (C); p < 0.0001. Uronic acid quantification and phagocytosis assay are presented as the mean ± standard deviation of three independent experiments. Kmg K. michiganensis, hvKpn hypervirulent K. pneumoniae, non-hmv Kpn non-hypermucoviscous K. pneumoniae, PBS Phosphate-buffered saline