Fig. 2
From: Enzyme kinetics of deoxyuridine triphosphatase from Western corn rootworm
Purification, quaternary structure, and enzyme activities of WCR dUTPase. a 18% SDS-PAGE. The molecular weights of 6-histidine-tagged dUTPase and dUTPase after being cleaved by thrombin are 17.5 and 15.6 kDa, respectively. b Size-exclusion chromatography. The estimated molecular weight of tag-free dUTPase was 48 kDa. c Hydrolysis of dUTP by dUTPase. Gray dots indicate the observed drop in absorbance and the predicted regression line obtained from a corresponding scan. The inset indicates linear transformation of the data between the arrows according to the integrated MichaelisโMenten equation and the corresponding regression line. dโi. Substrate specificity of WCR dUTPase. In each plot, the orange and blue lines show when a particular substrate is added and no enzyme is added, respectively. The substrates used are dUTP (d and e), dATP (f). dTTP (g), dCTP (h), and dGTP (i). The enzyme is preincubated with 5-mM EDTA in e