Fig. 2

Engineering the position specificity of CCoAOMT7. A Homology-based model of CCoAOMT7 illustrating the interaction of hesperetin with the active site of the wild type (WT) enzyme and the A50Y mutant. Hesperetin carbon atoms are shown in orange, and active site residue sidechains are depicted as red sticks. The protein fold is represented in blue. The carbon atoms of the cofactor SAH and the calcium ion are illustrated in green. In the mutant, the carbon atoms of the Tyr50 residue are highlighted in grey, and the hydrogen bonds are indicated by dashed lines. Active site residues are labelled. B Ratios of hesperetin to homoeriodictyol in the three mutants and the WT CCoAOMT7. C Hesperetin and homoeriodictyol titres in the three mutants and the WT CCoAOMT7. Error bars represent standard deviations of three biological replicates. Strains were grown in the presence of IPTG