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Fig. 2 | BMC Research Notes

Fig. 2

From: Placental extract suppresses lipid droplet accumulation by autophagy during the differentiation of adipose-derived mesenchymal stromal/stem cells into mature adipocytes

Fig. 2

PPE induced the expression of Lipa in ASC-differentiated adipocytes The gene expression of (A) adipocyte differentiation markers (Pparg, Cebpa, and Adipoq), (B) adipogenesis-related genes (Dgat1, Dgat2, Fasn, Soat1, and Soat2), and (C) lipolysis-related genes (Pnpla2, Abhd5, and Lipa) in ASCs cultured for 8 days with 1.0 mg/mL PPE were analyzed using RT-qPCR. Control represents cells cultured without PPE, and undifferentiation represents cells cultured without PPE and differentiation-inducing agents. The gene expression of adipocyte differentiation markers is presented relative to the value of those from the undifferentiation cells, and the expression of adipogenesis-related genes and lipolysis-related genes are presented relative to the value in Control. All RT-qPCR experiments were performed in triplicate, and the data are presented as the mean ± SEM. *p < 0.05, ***p < 0.001 (A: vs. undifferentiation, B and C: vs. Control.)

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BMC Research Notes

ISSN: 1756-0500

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