Fig. 3

Confirming specific targeting effects of P24E1102 A Rats administered with the negative control isotype antibody may show decreased severity scores after exposure to IMQ and may induce activation of non-antigen specific Fc receptors interactions. Therefore, we made F(ab)’2 antibody fragments A and retested them in the IMQ rat model. B Target specific attenuation of IMQ-induced scratching activity with P24E1102 F(ab)’2 30 mg/kg IV similar to the whole IgG molecule, while the isotype F(ab)’2 showed no targeting effect (n = 5/group). C IMQ induces scratching activity, but not biting or licking. Data is expressed as the mean (SEM). Statistical significance was determined using Student’s t test followed by Mann–Whitney post-test