Table 2 Detailed information about the multiplex PCR primers and probes and annealing temperature
Name | Sequence | Annealing temperature (°C) |
|---|---|---|
MTHFR-1298-F | TGGTCAGCTCCTCCCCTACA | 60 |
MTHFR-1298-R | CACTTATCATTATCTTCGCACAGACG | |
MTHFR-1298-Probe | Bio-ACCATTCCGGTTTGGTTCTCCC | |
MTHFR-677-F | TTTGGGAAATCCCGAGTCAA | |
MTHFR-677-R | TCTCCTTCCACGACGGAGGT | |
MTHFR-677-Probe | Bio-GCGGAAGAATGTGTCAGCCTC | |
PAI-1-F | TGCGAGCGATACGAAGTTCT | 55 |
PAI-1-R | GAGAGTCACTTTTATTGGGAACCA | |
PAI-1-Probe | Bio-CCGTGAAAGAATTATTTTTGTGTTTC | |
Factor V Leiden-F | GAGTCCTGCTACAAGATTTCA | 55 |
Factor V Leiden-R | CTGTCCAGGGATCTGCTCTT | |
Factor V Leiden-Probe | Bio-CCTCTGGGCTAATAGGACTACTTCTAATCTG |