Table 3 Master mix amplification of PCR reaction; component, concentration and volume
Component | Volume per reaction (μl) | Final concentration |
|---|---|---|
Template DNA | 1 | |
Each of the primers for MTHFR, PAI-1 and Factor V Leiden | 1 | 0.39 pmol |
2 × Master Mix Red | 12.5 | 1X |
H2O | 5.5 | |
Total volume | 25 |