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Table 4 Sanger Sequencing PCR reaction setup components

From: Spontaneous miscarriage driven by maternal genetic mutation at position of PAI-1-844G/A: shed light on a race-specific genetic polymorphism

Component

Volume per reaction (μl)

Buffer big dye

3

Big-dye enzyme (BDT)

3

Primer

0.33

DW

7.67

DNA

6