Spontaneous miscarriage driven by maternal genetic mutation at position of PAI-1-844G/A: shed light on a race-specific genetic polymorphism

Table 5 Sanger Sequencing PCR thermal profile

Cycles	Step	Temperature (°C)	Time
1 cycle	Initial Denaturation	94	5 min
	Denaturation	95	15 S
35 cycle	Annealing:
	MTHFR	60	30 S
	PAI-1	55	45 S
	Factor V Leiden	55	40 S
	Extension	72	45 S
	Final Extension	72	10 min
	Hold	4

ISSN: 1756-0500