Fig. 1

Yeast two hybrid and immunoprecipitations reveal a direct interaction between centriolin and HectD1. A Schematic shows the N-terminal region (amino acids 1–250) of the centriolin protein that was fused to the DNA binding domain (DBD) of the pGBKT7 vector. This construct was used in a yeast two hybrid screen that revealed an interaction between centriolin and HectD1. B Hela cells were arrested in the indicated cell cycle stages and cell lysates were subjected to immunoprecipitation using antibodies for centriolin. Western blot was then performed on immunoprecipitates using either HectD1 antibodies or centriolin antibodies