Fig. 2
Enhancer activity pattern in olf413SG1.1 enhancer trap strain and olf413MI02014 as depicted by reporter GFP expression. UAS-GFP strain was crossed with the two olf413 strains and the reporter GFP expression was analysed. A Embryo (stage 16) of olf413SG1.1 shows an expression in the ventral nerve cord region, in ventral (VC), lateral (LC) and dorsal (DC) neuronal clusters of nervous system. B and C Stage 12 and stage 17 embryos of olf413SG1.1 respectively showing the strong expression of GFP in the developing ventral nerve cord (VNC). D Stage 16 embryo of olf413MI02014-GAL4/UAS-GFP showing expression pattern similar to olf413SG1.1. E and F Stage 12 and stage 17 embryos of olf413MI02014 respectively with a very prominent reporter expression in the somatic muscle precursors (MPC) and Somatic muscle bundles (SM). G and H 72 h after larval hatched (ALH) (late 3rd instar larva) staged brain of olf413SG1.1 and olf413MI02014 strains respectively shows the reporter gene expression in clusters of neurons in central brain, suboesophageal ganglion (SOG) and Kenyon cells (KC). Ventral ganglion has the expression in centrointermedial neurons (CIMN) and the dorsolateral neurons (DLN) of individual segmental neuromeres. I and J Frontal view of adult brain (101 h APF) of olf413SG1.1 and olf413MI02014 respectively showing lobular plate region. The vertical system neurons of the optomotor responsive system [25] (ORS) shows strong enhanced activity in both the strains. All embryos have anterior to the left and the posterior to the right. K–M Brains at 72 h ALH, stained with Anti-Elav antibody (L, Red) marked with olf413SG1.1 GFP (K, green). M merged image showing that olf413SG1.1 cells are Elav positive. N–P Brains at 72 h ALH stained with Anti- Repo antibody (O, Red) and marked with olf413SG1.1 GFP (N, green). P merged image showing that olf413SG1.1 cells do not overlap with Repo positive cells. Q Represents the results of LC–MS/MS analysis for octopamine quantification. The bar graphs represent the mean ± SEM of octopamine levels (n = 3) quantified in head samples for homozygous olf413MI02014 with Oregon-K as control. A, B, D, E, F are lateral views, C is ventral view. Scale bar in Fig A-J represents 50 μm and in (K–P) represents 10 µm