Investigating the relationship between carbapenemase production and biofilm formation in Klebsiella pneumoniae clinical isolates

Table 1 Primers used for detection of carbapenemase genes

Gene	Primer DNA sequence	Amplicon size (bp)	Reference
bla_NDM−1	F-5’GGTTTGGCGATCTGGTTTTC 3’ R-5’CGGAATGGCTCATCACGATC 3’	621 bp	[15]
bla_KPC	F-5’CGTCTAGTTCTGCTGTCTTG 3’ R-5’CTTGTCATCCTTGTTAGGCG3’	798 bp	[15]
bla_OXA−48	F-5’GCGTGGTTAAGGATGAACA3’ R-5’CATCAAGTTCAACCCAACCG3’	438 bp	[15]
bla_IMP	F-5’GGAATAGAGTGGCTTAAYTCTC3’ R-5’GGTTTAAYAAAACAACCACC3’	232 bp	[16]
bla_SPM	F-5’AAAATCTGGGTACGCAAACG3’ R-5’ACATTATCCGCTGGAACAGG3’	271 bp	[16]
bla_VIM	F-5’GATGGTGTTTGGTCGCATA3’ R-5’CGAATGCGCAGCACCAG3’	390 bp	[16]

ISSN: 1756-0500