Fig. 3
Effect of LPS on proinflammatory cytokine gene expression. (A) TNF mRNA, (B) COX2 mRNA, (C) GM-CSF mRNA, (D) IFNγ mRNA, (E) IL12b mRNA. RAW264.7 macrophages were treated with LPS for 2–24 h. The data represent the mean and standard deviation of three independent samples. “*” and “**” displayed in the Fig. represent significant difference between the control and the treatment at p < 0.05 and p < 0.01, respectively. The “CT” values of each gene in untreated cells were 17.82 ± 0.81 (Rpl32 control), 29.19 ± 2.68 (Tnf), 30.15 ± 2.38 (Cox2), 27.58 ± 2.06 (Gm-csf), 27.70 ± 2.42 (Infr) and 28.03 ± 1.89 (Il12b)