Table 1 Methylobacterium Strains and Plasmids Used in This Study

AM1-W

crtI::ISphoA/hah (i.e. crtI⁵⁰²) strain; white

[14]

C2925H

Cm^R, Sm^R;

New England Biolabs

CM253.1

ΔmptG

[18]

CM501

Isolate of wild-type M. extorquens AM1; pink

This study

CM502

Isolate of AM1-W; white "wild-type" with crtI⁵⁰² allele

This study

CM508

Isolate of ΔmptG strain CM253.1

This study

CM1122

Km^R; ΔhprA::kan; pCM431 integrated into CM501

This study

CM1123

Km^R; ΔhprA::kan crtI⁵⁰²; pCM431 integrated into CM502

This study

CM1203

ΔhprA; kan deleted from CM1122

This study

CM1204

ΔhprA crtI⁵⁰²; kan deleted from CM1123

This study

CM1263

Tc^R, sucrose^S; pCM441 integrated into CM501; crtI⁵⁰² upstream; white

This study

CM1264

Tc^R, sucrose^S; pCM441 integrated into CM501; crtI upstream; pink

This study

CM1265

Tc^R, sucrose^S; pCM440 integrated into CM502; crtI upstream; pink

This study

CM1266

Tc^R, sucrose^S; pCM440 integrated into CM502; crtI⁵⁰² upstream; white

This study

M. extorquens AM1

Rif^R isolate; pink

[9]

Plasmids

pCM157

Tc^R; broad-host-range cre expression vector

[4]

pCM184

Ap^R, Km^R, Tc^R; broad-host range cre-lox allelic exchange vector

[4]

pCM411

Ap^R, Km^R; mptG region cloned into pCR2.1

This study

pCM417

Ap^R, Km^R; crtI region cloned into pCR2.1

This study

pCM424

Ap^R, Km^R; ΔmptG region cloned into pCR2.1

This study

pCM426

Ap^R, Km^R; crtI⁵⁰² region cloned into pCR2.1

This study

pCM428

Ap^R, Km^R; pCR2.1 with hprA upstream flank

This study

pCM429

Ap^R, Km^R; pCR2.1 with hprA downstream flank

This study

pCM430

Ap^R, Km^R, Tc^R; pCM184 with hprA upstream flank from pCM428

This study

pCM431

Ap^R, Km^R, Tc^R; pCM430 with hprA downstream flank from pCM429

This study

pCM432

Ap^R, Tc^R; synthetic linker introduced into pCM184

This study

pCM433

Ap^R, Cm^R, Tc^R; pCM432 with sacB-cat fragment from pDS132; broad-host-range sacB-based allelic exchange vector

This study

pCM433r

Ap^R, Cm^R, Tc^R; pCM433 with sacB-cat fragment in the opposite orientation as in pCM433

This study

pCM434

Ap^R, Km^R; hprA region cloned into pCR2.1

This study

pCM435

Ap^R, Cm^R, Tc^R; hprA region from pCM434 cloned into pCM433

This study

pCM436

Ap^R, Cm^R, Tc^R; mptG region from pCM411 cloned into pCM433

This study

pCM437

Ap^R, Cm^R, Tc^R; ΔmptG region from pCM424 cloned into pCM433

This study

pCM438

Ap^R, Km^R; ΔhprA region cloned into pCR2.1

This study

pCM439

Ap^R, Cm^R, Tc^R; ΔhprA region from pCM438 cloned into pCM433

This study

pCM440

Ap^R, Cm^R, Tc^R; crtI region from pCM417 cloned into pCM433

This study

pCM441

Ap^R, Cm^R, Tc^R; crtI⁵⁰² region from pCM426 cloned into pCM433

This study

pCR2.1

Ap^R, Km^R; PCR cloning vector

Invitrogen

pDS132

Ap^R, Cm^R;, sacB-based allelic exchange vector with λpir-dependent R6Kori

[10]

pRK2073

Sm^R; helper plasmid supplying IncP tra functions

[17]