a. Predicted secondary structure of the wild-type EBER2 RNA. The 5'3' stem ("S"), and the two loops ("L1", "L2"), as well as the XmaI cloning site used to construct the EBER2-L2 mutant are indicated. b. Alternate predicted secondary structures for the EBER2-L2 RNA are illustrated. Labeling as in (a). Both structures differ minimally in their free energy (ΔG = 3.1 kJ). c. Northern blot analysis of transfected HEK293 cells confirming the expression of both EBER2 constructs at comparable levels.