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Table 2 Primer Sequences of the Studied Genes

From: Genetic profile of Egyptian hepatocellular-carcinoma associated with hepatitis C virus Genotype 4 by 15 K cDNA microarray: Preliminary study

Gene name Primer sequence Annealing temp.
B-actin s:5'-ACA CTG TGC CCA ACG AGG-3'
as:5'-AGG GGC CGG TCA TAC T-3'
55–59
NOTCH4 s:5'-GAG GAC AGC ATT GGT CTC AAG G-3'
as:5'-CAA CTC CAT CCT CAT CAA CTT CTG-3'
60.4
VISA s:5'-TGC CGT TTG CTG AAG ACA A-3'
as:5'-TTC GTC CGC GAG ATC AAC T-3'
56.8
CDK-7 s:5'-CGG GCT TTA CGG CGC CGG ATG G-3'
as:5'-CCC TCA GTA GTA AAA TGT TGT CC-3'
60
ISGF3G s:5'-CTG GCA CAT GGC ACA CAC-3'
as:5'-CAT CAA AGC GAC AGC ACA GT-3'
59
TNF-α s:5'-ACA AGC CTG TAG CCC ATG TT-3'
as:5'-AAA GTA GAC CTG CCC AGA CT-3'
57.9
BAX s:5'-CAT GGA ACT GAT GAT GAT GAA-3'
as:5'-CTC CAA CGA AAA ATG ATA-3'
60