Table 1 Primers used for sequencing and genotyping
From: Mapping of the spontaneous deletion in the Ap3d1 gene of mocha mice: fast and reliable genotyping
Primer | Sequence | Start | End |
|---|---|---|---|
Mocha F1 | 5'- CCT CAA GCC CGT CAG AAG- 3' | 5322 | 5339 |
Intron1 F | 5'- TTG AAC TCG GAG ACA TCC TCT GC- 3' | 5616 | 5638 |
Mocha F2 | 5'- CTG GGT GGC ATT TAG TTA CTT GAG GC- 3' | 7790 | 7815 |
Mocha R1 | 5'- CTT CTG GGC TGG CTG GAT GTA AG- 3' | 15485 | 15507 |
Intron7 R | 5'- CCA ATA CCA ACC AAC CAA CAA CC- 3' | 17473 | 17495 |
Mocha R2 | 5'- TGA CAT TAA CAG CCG CAG AC- 3' | 18391 | 18410 |
Genotyping primers | |||
Mocha F | 5'- CCT GCT TCT GAG TGC TTG GG- 3' | 6193 | 6212 |
WT F | 5'- GGC TGC TTC TGG ACT GTT CG- 3' | 17006 | 17025 |
Common R | 5'- CTT CTC CAC CCT CTT TCC ACC C- 3' | 17661 | 17682 |