Skip to main content

Advertisement

Figure 1 | BMC Research Notes

Figure 1

From: Probing of Actinobacillus pleuropneumoniae ApxIIIA toxin-dependent cytotoxicity towards mammalian peripheral blood mononucleated cells

Figure 1

A. Spreading of porcine PBMCs elements in SSC/FSC dot plot after rApxIIIA addition. P1 (red) and P2 (blue) populations represent mock- (RPMI-1640) and rApxIIIA-exposed porcine PBMCs respectively. X-axis and Y-axis represent FSC and SSC values respectively. B. Surface expression of LFA-1 by mock- (P1, red) and rApxIIIA (P2, blue) exposed porcine PBMCs. Surface labeling was made with anti-PoCD18 mAb MCA1972. Labeling with an isotype-matched nonpertinent murine mAb was used as negative control (black). X-axis shows fluorescence intensity (Alexa 488) and the Y-axis represents cell count. The percentage of Alexa 488-positive cells is indicated within the panels. C. Spreading of porcine PBMCs elements in PI/FSC dot plot after rApxIIIA addition. P1 (red) and P2 (blue) populations represent mock- and rApxIIIA-exposed porcine PBMCs respectively. Two subpopulations can be observed for P2: the first shows an intermediate labeling (P2A) while the second (P2B) is very strongly tagged, corresponding to intermediate and final stages of cell death respectively. The bar represents the positivity threshold. X-axis and Y-axis represent FSC and PI-fluorescence values. D. Distribution of PI labeling among mock- (red) and rApxIIIA-exposed (blue) porcine PBMCs. The P2A and P2B subpopulations are readily detected. X-axis shows fluorescence intensity (PI) and the Y-axis represents cell count. Percentage of PI-positive cells is indicated within the panels.

Back to article page