Figure 1

A. Spreading of porcine PBMCs elements in SSC/FSC dot plot after rApxIIIA addition. P₁ (red) and P₂ (blue) populations represent mock- (RPMI-1640) and rApxIIIA-exposed porcine PBMCs respectively. X-axis and Y-axis represent FSC and SSC values respectively. B. Surface expression of LFA-1 by mock- (P₁, red) and rApxIIIA (P₂, blue) exposed porcine PBMCs. Surface labeling was made with anti-PoCD18 mAb MCA1972. Labeling with an isotype-matched nonpertinent murine mAb was used as negative control (black). X-axis shows fluorescence intensity (Alexa 488) and the Y-axis represents cell count. The percentage of Alexa 488-positive cells is indicated within the panels. C. Spreading of porcine PBMCs elements in PI/FSC dot plot after rApxIIIA addition. P₁ (red) and P₂ (blue) populations represent mock- and rApxIIIA-exposed porcine PBMCs respectively. Two subpopulations can be observed for P₂: the first shows an intermediate labeling (P₂A) while the second (P₂B) is very strongly tagged, corresponding to intermediate and final stages of cell death respectively. The bar represents the positivity threshold. X-axis and Y-axis represent FSC and PI-fluorescence values. D. Distribution of PI labeling among mock- (red) and rApxIIIA-exposed (blue) porcine PBMCs. The P₂A and P₂B subpopulations are readily detected. X-axis shows fluorescence intensity (PI) and the Y-axis represents cell count. Percentage of PI-positive cells is indicated within the panels.