Figure 2

Kinetics of porcine PBMCs death upon exposition to rApxIIIA crude toxin preparation. Kinetics of the cytotoxic action was characterized by incubating porcine PBMCs with 0.31 μg rApxIIIA crude toxin preparation and stopping the exposure after 1, 10, 20, 30, 45, 60, 90 and 120 minutes. Horizontal axis, duration of exposition; vertical axis, fraction of PI-positive PBMCs. Basal cell death rate was measured from mock-exposed (RPMI-1640) PBMCs for comparison. Values are means ± SDs from three representative experiments.