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Table 2 TILLING analysis for MtPHY1 and the putative M. truncatula orthologue of MsTI from Medicago scutellata

From: An Italian functional genomic resource for Medicago truncatula

Gene GenBank Primer pair No. of mutations Type of mutation
MtPHY1 AY878355 5'-AACAATGCCGGTTTCAGGTC
5'-TTGAAGGCGCAGAAACCTCT
8 C → T (4)
G → A (4)
Trypsin inhibitor AC135311 5'-TGTTTGTTTCGTCTGGAGCA
5'-GTGGACGGTCTCCCAGACTC
7 C → T (2)
G → A (5)