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Figure 1 | BMC Research Notes

Figure 1

From: Interaction of the growth and tumour suppressor NORE1A with microtubules is not required for its growth-suppressive function

Figure 1

Interaction of C19ORF5 and MAP1B proteins with wild type and mutant NORE1A. A-C: HEK293 cells were transfected with FLAG-tagged wild type NORE1A, fragments of NORE1A, or deletion mutants as indicated. Cell lysates were precipitated for FLAG and the precipitate was probed with antibody against C19ORF5 and MAP1B (upper panels). Lower panels show expression of FLAG-tagged proteins in cell extracts. Asterisk denotes NORE1A 1–268 degradation product. D: A549 cells were infected with retrovirus coding for hrGFP-tagged wild type NORE1A or hrGFP alone as indicated. Cell extracts were precipitated for NORE1A and the precipitate was probed with antibody against MAP1B (upper panel). Lower panels show expression of NORE1A and MAP1B in cell extracts.

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