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Figure 4 | BMC Research Notes

Figure 4

From: Interaction of the growth and tumour suppressor NORE1A with microtubules is not required for its growth-suppressive function

Figure 4

The MAP1B protein is required for NORE1A binding to microtubules. A, B: A549 cells were co-transfected with RNA oligos labelled with the Cy3 dye and anti-MAP1B siRNAs or control siRNA. After one day cells were infected with retrovirus encoding GFP-NORE1A. Two days later, cells were sorted using GFP and Cy3 markers, allowed to recover overnight, lysed and equal amounts of cell extract were probed for expression of MAP1B protein (A, upper panel) and GFP-NORE1A expression level (A, lower panel). Lysates of GFP and Cy3- positive cells were examined for the ability of GFP-NORE1A to interact with microtubules as described in Figure 1 (B). C: A549 cells expressing GFP-NORE1A were transfected with anti-MAP1B siRNA pool or control siRNA. Cells were fixed, processed for immunofluorescence, and imaged as described in Methods. Each panel shows NORE1A, microtubules (stained with α-tubulin), centrosomes (stained with pericentrin) and a superimposed image. Arrows indicate centrosomes. Bar, 10 μm.

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