Figure 3

Selected genes which might be related to disease progression from correlation analysis and RT-PCR validation. Genes whose expression increased or decreased with disease progression were identified by correlation analysis of microarray data (A~E). Changes in transcript abundance are expressed as amount of change relative to control mean for that gene, and individual values of three different mice and the mean are shown at each week. (F~J) Validation of the microarray results by QRT-PCR analysis, showing relative change of PCR signal between wild-type and Pkd1^{L 3/L 3}mice (mean ± SD of triplicate PCR reactions of pooled RNA; n = 3; *, P < 0.05; **, P < 0.01 compared with wild-type mice, by Student's t-test).