Table 1 Primer pairs used for real time quantitative RT-PCR
Gene abbreviation | Gene definition | GenBank accession | Unigene ID | Primer pair (5'-forward-3'/5'-reverse-3') | Product size (bp) |
|---|---|---|---|---|---|
STS1 | Stilbene synthase1 | DQ366301 | Vvi.8 | CGAAGCAACTAGGCATGTGT/CTCCCCAATCCAATCCTTCA | 134 |
STS2 | Stilbene synthase2 | DQ366302 | Vvi.8 | ACCAAAGTCCAAGATCACCCA/ACAACATCACCCTTCTAACCGAT | 122 |
PAL1 | Phenylalanine ammonia lyase | EC987386 | Vvi.1950 | CCGAACCGAATCAAGGACTG/GTTCCAGCCACTGAGACAAT | 183 |
C4H1 | Cinnamate-4-hydroxylse | EC995763 | Vvi.6228 | AAAGGGTGGGCAGTTCAGTT/GGGGGGTGAAAGGAAGATAT | 109 |
4CL1 | 4-coumarate-CoA ligase | EC947790 | Vvi.1251 | CTGATGCCGCTGTTGTTTCG/GCAGGATTTTACCCGATGGA | 198 |
CHS1 | Chalcone synthase1 | EC996578 | Vvi.117 | GTCCCAGGGTTGATTTCCAA/TCTCTTCCTTCAGACCCAGTT | 157 |
CHS2 | Chalcone synthase2 | EC996527 | Vvi.1973 | TTTGGGCATCAAGGACTGGA/CTCGGGCTTTAGGGCTAAT | 100 |
CCR2 | Cinnamoyl-CoA reductase | CF517687 | Vvi.15864 | ACAGCATGACGACTCTCTTCG/AGTGACAAGGGGTGGATTGA | 182 |