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Figure 1 | BMC Research Notes

Figure 1

From: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination

Figure 1

General outline of the antibiotic genes introduction via recombination. pINS plasmids (pINS-Blast, pINS-Puro and pINS-Neo) produced in the pir+ E. coli strains can be integrated via Cre-mediated recombination into any of the target vectors (phrGFP, pT-FLAG, pT-TK and pT-BS) produced in the pir- E. coli strains. The recombination mix is transformed into the pir- E. coli strain and the recombinant plasmid is selected by Kan or Cam markers provided by pINS vector.

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