Functionality of Blast, Puro and G418-resistance genes from pINS plasmids is preserved in the products of recombination. Left part. Colony forming assay. HeLa cells transfected by 1 mkg of indicated plasmids were selected by either blasticidin S, puromycin or G418. After completion of selection the cells were stained by methylene blue. Insertion vectors pINS-Blast, pINS-Neo and pINS-Puro and the products of their recombination with Target vector phrGFP (Blast × GFP, Neo × GFP and Puro × GFP) provide HeLa cells with the resistance to blasticidin S, G418 or puromycin respectively. Right part. Schematic representation of the Cre-mediated recombination between Insertion vectors pINS-Blast, pINS-Neo and pINS-Puro and Target vector phrGFP.