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Figure 1 | BMC Research Notes

Figure 1

From: Bipotential mouse embryonic liver (BMEL) cells spontaneously express Pdx1 and Ngn3 but do not undergo further pancreatic differentiation upon Hes1 down-regulation

Figure 1

BMEL characteristics in adherent vs aggregate culture. (A) Morphology of BMEL cells grown for 4 days in (i) adherent or (ii) floating aggregate culture. (B) Western blot analysis of BMEL cells after four days of adherent or aggregate culture showing the expression of a 46kDa Pdx1 protein (n = 3). (C) RT-PCR profiles of adherent (Adh.) or aggregate (Agg) BMEL cells (n = 5). * Three out of five aggregate samples yielded positive ngn3 RT-PCR signals. cDNA from adult mouse pancreas (Panc) served as positive control for all genes except for Ngn3 for which a plasmid containing the ngn3 open reading frame was used as positive control. (D) Spontaneous Pdx1, Ngn3, and Hes1 expression located predominantly in the nuclei of adherent (top row) or aggregate (bottom row) BMEL cells. Scale bars = 40 μm.

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