Table 1 Primers and RT-PCR conditions
Primers employed in the RT-PCR | RT-PCRs performed | ||||
|---|---|---|---|---|---|
Primer | Sequence (5'->3') | GLB1 Exona | Transcript specificity | Pairs of primers employed | AT (°C) |
C1F | ACTGCAGAGCCGGGAGGCTGGT | 1 | EBP/β-Gal | C1F/EBPR | 58 |
C3R | CCAAAGTGACCTTTCCATATG | 11 | EBP/β-Gal | EBPF/C3R | 56.5 |
EBPF | GGCTGAACGCCATCCAGACATT | 2–5 | EBP | T7/SP6 | 55 |
EBPR | TGATGTTGCTGCCTGCACTG | 7–5 | EBP | T7/EBPR | 56.5 |
2_4F | ACGCCATCCAGACGGAGGAT | 2–4 | NN | EBPF/SP6 | 58 |
4_6F | TCCTCCGACCCAGGTTGAA | 4–6 | NN | 2_4F/C3R | 59 |
T7 | TAATACGACTCACTATAGGG | -- | pcDNA3 | 2_4F/SP6 | 58.3 |
SP6 | GATTTAGGTGACACTATAG | -- | pcDNA3 | 4_6F/C3R | 59 |
C1Fm | GAGACCCCATCGTGGCGCGA | 1 | m EBP/β-Gal | C1Fm/C1Rm | 59 |
C1Rm | CTCTAGTAGCCAAGCAGGTAAGC | 4 | m β-Gal | EBPFm/EBPRm | 58 |
EBPFm | GGGCTGAATGCTATCCAGATAC | 2–5 | m EBP | ||
EBPRm | TGTGATATTGTTGCCTGCACGGT | 7–5 | m EBP | ||