Table 1 Details on exon-spanning primers used for splice variant detection.
Primer name | Primer sequence (5'→3') | Location | Amplicon length |
|---|---|---|---|
PGC1A+Ex1,3 | CATGTGCAACCAGGACTCTGT | Exon 1 | 294 bp |
PGC1A-Ex1,3 | TCTTCATCCACAGGGAGACTG | Exon 3 | |
PGC1A+Ex2,4 | TTCTGGGTGGACTCAAGTGG | Exon 2 | 367 bp |
PGC1A-Ex2,4 | TTGTGGTTTGCATGGTTCTG | Exon 4 | |
PGC1A+Ex3,5 | CCCTGTGGATGAAGACGGATT | Exon 3 | 367 bp |
PGC1A-Ex3,5 | AGGAGGGTCATCATTTGTGGT | Exon 5 | |
PGC1A+E4,6 | CAGAACCATGCAAACCACAA | Exon 4 | 296 bp |
PGC1A-Ex4,6 | TCTGGGGTCAGAGGAAGAGAT | Exon 6 | |
PGC1A+Ex5,7 | CAACAGCAAAAGCCACAAAGA | Exon 5 | 284 bp |
PGC1A-Ex5,7 | CAGTTCCAGAGAGTTCC | Exon 7 | |
PGC1A+Ex6,8 | ATCTCTTCCTCTGACCCCAGA | Exon 6 | 266 bp |
PGC1A-Ex6,8 | TCTTGGTGGAGTTGTTGCC | Exon 8 | |
PGC1A+E7,9 | TGTGGAACTCTCTGGAACTGC | Exon 7 | 1017 bp |
PGC1A-Ex7,9 | GAACGTGATCTGGCGCAC | Exon 9 | |
PGC1A+E8,10 | TTCCGTATCACCACCCAAA | Exon 8 | 298 bp |
PGC1A-Ex8,10 | TTCCCTCTTCAGCCTCTCG | Exon 10 | |
PGC1A+Ex9,11 | TACTCTGAGTCAGGCCACTGC | Exon 9 | 302 bp |
PGC1A-Ex9,11 | TCACCAAAAACTTCAAAACGG | Exon 11 | |
PGC1A+Ex10,12 | CGAGAGGCTGAAGAGGGAA | Exon 10 | 267 bp |
PGC1A-Ex10,12 | GCAGCAAAAGCATCACAGG | Exon 12 | |
PGC1A+Ex11,13 | AGGGACCGTTTTGAAGTTTTT | Exon 11 | 255 bp |
PGC1A-Ex11,13 | GCTCTTGGTGGAAGCAGGA | Exon 13 |