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Table 2 Comparative results of Direct Fluorescent Antibody Test (DFA), Mouse Inoculation Test (MIT), Reverse-transcriptase Polymerase Chain Reaction (RT-PCR) and Heminested RT-PCR techniques for rabies diagnosis in thawed and decomposed positive samples.

From: Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples

     Thawed Decomposed
Samples Specie DFA MIT RT-PCR hnRT-PCR RT-PCR hnRT-PCR
220-04 Bov + + - - - -
210-04 Bov + + - + - +
218-04 Bov + + + + - +
139-04 Bov - + + + IM IM
107-04 Eq + + + + IM IM
R-5473-00 Bov + + + + + +
R-442-01 Bov + + - + - +
R-298-01 Eq + + - + - +
R-1762-01 Bov + + + + + +
R-366-01 Bov + + + + - -
R-318-01 Bov + + - - - -
R-1760-01 Bov + + - + - +
R-370-01 Bov + + + + - -
R-3386-01 Bov + + - + - -
R-2210-01 Eq + + + + + +
R-3499-01 Bov + + + + + +
R-1001-01 Bov + + - - - -
R-4938-01 Bov + + + + + +
R-441-01 Bov + + - + - -
R-404-01 Bov + + + + + +
R-3388-99 Bov + + + + + +
R-5070-01 Bov + + - + - +
R-207-01 Bov + + + + + +
R-1779-01 Eq + + + + + +
R-5159-01 Bov + + - - - -
R-027-02 Eq + + - + - +
R-2167-01 Eq + + + + - +
R-2575-03 Eq + + + + - +
R-0081-02 Eq + + + + + +
R-0297-01 Eq + + - + - +
R-785-01 Eq + + - + - +
R-3942-02 Eq + + + + + +
R-7124-03 Eq + + + + - +
R-511-02 Bov + + + + + +
R-248-02 Eq + + - + - +
R-235-02 Eq + + - + - +
R-1831-01 Eq + + - + - +
R-3805-02 Bov + + - + - -
R-6429-02 Eq + + - - - -
R-119-02 Bov + + - + - -
R-120-02 Bov + + + + - +
R-299-01 Eq + + + + + +
R-367-01 Bov + + + + + +
R-5093-01 Bov + + + + + +
R-5160-01 Bov + + - + - +
R-4065-01 Bov + + - + - +
R-2056-01 Bov + + + + + +
R-4286-01 Bov + + - + - -
R-6558-02 Eq + + - + - +
515-05 Bov + + + + + +
  1. IM = Insuficient Material DFA/MIT: previously evaluated Samples in bold: false negative results in decomposed materials Bov: bovines Eq: equines
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