Discovering and verifying DNA polymorphisms in a mung bean [V. radiata(L.) R. Wilczek] collection by EcoTILLING and sequencing

Table 2 List of primers

Name	Target	Forward	Reverse	MgCl₂ (mM)	AT_m (°C)
BTF3b	IS	TCAAAAGTCTCCCCGGGGACAAGA	CCAAAGTACAAGCATCTATTGCTGCCA	4.50	61
CDC2	IS	CAACTTTGCAAGGGTGTTGCTTTCT	ACTAACACCTGGCCACACATCTTCA	4.25	65
BP1	unknown	GTTATGGAGTTGATGAGAGGTGTCAGATA	TTGGTAAGTTCTGGAAAATGCCAACCATA	3.75	65
AIGP	IS	CTGATAGGGCCAGGAGGCAGGGAAGA	GTTTTTTAGCATTTGGACGAATGGTTGGT	3.75	60
ATCP	IS	AACCAATTGGTATTGCAGCTCAGAGCCA	TTCCTTGCCAAGAACAAACCGAATGTCA	3.75	65
CALTL	IS	GTGGAAGGCACCATTGATTGACAAC	TCTTCTTCTCAGCCTCTTCAAATGC	3.75	67
MSU380	IS	CACTCATTGCAATTTCCATGCTTCA	CAGTTGTTGTAGCAAGGGCACA	3.75	65
RL3B	unknown	GACACGGTTCTTTGGGATTTCTC	CCTGGCTTTTCGACTTCTCTGAC	3.75	63
DNABP	IS	CAAGACATGGCTCCAATGAG	AAGAGGTAGGCGCTTTTGTG	3.00	65
SHMT	IS	CCAAACAAGGAAAAGAGGTAA	TGACTTATTCACCCCATCCA	4.25	55

A list of the primer sequences including annealing temperature (AT_M) and final MgCl₂ concentration (mM) that were used for PCR in this study. The majority of the PCR fragments used are intron spanning (IS); however, some are unknown.

ISSN: 1756-0500