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Figure 1 | BMC Research Notes

Figure 1

From: Lactogenic differentiation of HC11 cells is not accompanied by downregulation of AP-2 transcription factor genes

Figure 1

Expression of AP-2 family members in HC11 cells. RNA from HC11 cells cultivated in EGF-containing medium (EGF) or in differentiation conditions (DIP) was subjected to RT-PCR using primers specific for the AP-2 isoforms indicated (upper panel) or milk protein genes (middle panel). Amplification of beta-actin served as a loading control (lower panel). Control reactions were carried out in the absence of reverse transcriptase (-RT). Positive controls (contr.) consisted of cloned PCR fragments (10 pg plasmid DNA) in the case of AP-2 isoforms, or of cDNA derived from a lactating mouse mammary gland for differentiation markers.

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