Figure 1
From: Biologically active fibroblast growth factor 1 tagged with various epitopes
Schematic diagram depicting the strategy for plasmid construction. Complementary DNAs encoding FGF1 and V5-(His)6 tag (a), myc-(His)6 tag (b) or 3xFLAG-(His)6 tag (c) were separately amplified, and the products were mixed and used as templates for overlap extension PCR. The sequences of the primers are summarized in Table 1.