Figure 1From: Biologically active fibroblast growth factor 1 tagged with various epitopesSchematic diagram depicting the strategy for plasmid construction. Complementary DNAs encoding FGF1 and V5-(His)6 tag (a), myc-(His)6 tag (b) or 3xFLAG-(His)6 tag (c) were separately amplified, and the products were mixed and used as templates for overlap extension PCR. The sequences of the primers are summarized in Table 1.Back to article page