Table 1 Primers used in this study

#127

forward

5'-gta ata cga ctc act ata ggg-3'

vector primer

#619

reverse

5'-acc ttc atc aga aga gac tgg cag-3'

FGF1/3' + V5-(His)₆/5'

#397

reverse

5'-cga gct cgg atc aga aga gac tgg cag-3'

FGF1/3' + myc-(His)₆/5'

#620

forward

5'-tct gat gaa ggt aag cct atc cct-3'

FGF1/3' + V5-(His)₆/5'

#301

reverse

5'-tag aag gca cag tcg agg-3'

vector primer

#395

forward

5'-cca gtc tct tct gat ccg agc tcg gta cca-3'

FGF1/3' + myc-(His)₆/5'

#396

reverse

5'-gct ctA GAT CTt caa tga tga tga tga tga tgg tc-3'

myc-(His)₆/3' + Bgl II

#468

forward

5'-cag ccg CTC GAG a-3'

Xho I + 3xFLAG-(His)₆/5'

#469

reverse

5'-tgc GGG CCC tca a-3'

3xFLAG-(His)₆/5' + Apa I

#466

forward

5'-ccg CTC GAG act aca aag acc atg acg gtg att ata aag atc atg aca tcg act aca ag-3'

megaprimer/5'

#467

reverse

5'-tgc GGG CCC tca atg gtg atg gtg atg atg acc ctt gtc atc gtc atc ctt gta gtc ga-3'

megaprimer/3'

#476

forward

5'-cgG AAT TCc cac cat gtc ccg ggg agc-3'

EcoR I + FGF1/5'

#477

reverse

5'-ccg CTC GAG cat cag aag aga ctg gca g-3'

FGF1/3' + Xho I

#117

forward

5'-tct tcc gat aga ctg cgt cg-3'

FGF1

#537

reverse

5'-gaA GAT CTc ttc aat ggt gat ggt gat gat gac c-3'

(His)₆/3' + Bgl II

#178

reverse

5'-agc ccg tcg gtg tcc atg gc-3'

FGF1