Schematic of Synthetic Universal Amplicon (SUA) construction. (a) An extension reaction using two oligonucleotides was used to generate a double stranded DNA product. (b) This product served as template for PCR amplification to attach a T7 RNA promoter sequence. (c) The 127 bp SUA was transcribed in vitro to RNA. (d) The RNA product was subsequently reverse transcribed into a fluorescent single stranded cDNA target.