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Table 1 Analysis of the microarray meter targets.

From: Development and application of a microarray meter tool to optimize microarray experiments

Control OD DNA A260 OD dye A650/550 DNA ng/ul DNA pmol/ul DYE pmol/ul Cy/DNA U/DNA % labeling efficiency
ybbR 0.294 0.322 9.692 0.048 1.288 26.76 50.00 53.53
ybaQ 0.221 0.162 7.277 0.037 0.648 17.73 65.25 27.18
ycxA 0.202 0.220 6.664 0.033 0.878 26.36 64.25 41.03
ybaS 0.219 0.223 7.213 0.038 0.891 23.44 69.00 33.97
ybaF 0.270 0.240 8.910 0.048 0.960 20.01 59.25 33.78
ybdO 0.189 0.201 6.223 0.039 0.806 20.92 38.00 55.05
ybaC 0.405 0.396 13.365 0.073 1.584 21.72 57.50 37.78
yacK 0.293 0.293 9.653 0.055 1.170 21.18 50.00 42.36
SUA 0.168 0.023 5.440 0.214 1.120 5.22 8.25 63.27
  1. The OD DNA and OD DYE fields list optical density data derived from the spectrophotometer for each target.
  2. Mass concentration (ng/ul), DNA molar concentration (pmol/ul), cyanine dye (DYE) molar concentrations (pmol/ul),
  3. Cy/DNA (ratio of the molecular dye and DNA concentration per target), U/DNA (expected amount of labeled uracil per DNA molecule), percentage labeling efficiency (ratio of expected versus measured labeled uridines per DNA molecule) were determined as outlined in the text.