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Table 3 Microarray meter probes

From: Development and application of a microarray meter tool to optimize microarray experiments

spot ID clone ID probe ng target pg
1 ybbR 200 2500
2 ybaQ 200 500
3 ycxA 200 100
4 ybaS 200 40
5 ybaF 200 20
6 ybdO 200 40
7 ybaC 200 0.20
8 yacK 200 0
9 blank 0 0
10 ybbR 20 2500
11 ybaQ 20 500
12 ycxA 20 100
13 ybaS 20 40
14 ybaF 20 20
15 ybdO 20 40
16 ybaC 20 0.20
17 yacK 20 0
18 blank 0 0
19 ybbR 2 2500
20 ybaQ 2 500
21 ycxA 2 100
22 ybaS 2 40
23 ybaF 2 20
24 ybdO 2 40
25 ybaC 2 0.20
26 yacK 2 0
27 blank 0 0
28 ycxA 50 100
29 ycxA 5 100
30 ycxA 0.5 100
31 ycxA 0.05 100
32 blank 0 0
  1. The relationship between each printed probe and the corresponding hybridized target is outlined.
  2. Serial dilutions were carried out so that each of the eight probes was diluted from DNA stocks at 200, 20 and 2 ng/l.
  3. One of the probes ycxA was also serially diluted to additional concentrations namely, 5 ng/l, 0.5 ng/l and 0.05 ng/l.
  4. The yacK probe was omitted from the microarray meter plate used with the QArrayMini and BioRobotics Microgrid II robots.