Development and application of a microarray meter tool to optimize microarray experiments

BMC Research Notes

Table 3 Microarray meter probes

spot ID	clone ID	probe ng	target pg
1	ybbR	200	2500
2	ybaQ	200	500
3	ycxA	200	100
4	ybaS	200	40
5	ybaF	200	20
6	ybdO	200	40
7	ybaC	200	0.20
8	yacK	200	0
9	blank	0	0
10	ybbR	20	2500
11	ybaQ	20	500
12	ycxA	20	100
13	ybaS	20	40
14	ybaF	20	20
15	ybdO	20	40
16	ybaC	20	0.20
17	yacK	20	0
18	blank	0	0
19	ybbR	2	2500
20	ybaQ	2	500
21	ycxA	2	100
22	ybaS	2	40
23	ybaF	2	20
24	ybdO	2	40
25	ybaC	2	0.20
26	yacK	2	0
27	blank	0	0
28	ycxA	50	100
29	ycxA	5	100
30	ycxA	0.5	100
31	ycxA	0.05	100
32	blank	0	0

The relationship between each printed probe and the corresponding hybridized target is outlined.
Serial dilutions were carried out so that each of the eight probes was diluted from DNA stocks at 200, 20 and 2 ng/l.
One of the probes ycxA was also serially diluted to additional concentrations namely, 5 ng/l, 0.5 ng/l and 0.05 ng/l.
The yacK probe was omitted from the microarray meter plate used with the QArrayMini and BioRobotics Microgrid II robots.

ISSN: 1756-0500