Streptococcus pneumoniaeearly response genes to human lung epithelial cells

BMC Research Notes

Table 1 Oligonucleotide primers used for qRT-PCR analysis

Gene name	TIGR4 genome acc. No.	Oligonucleotide primers 5' to 3'	Amplified product (bp)
purH	SP0050	Sense: TCAAGCAACCAATGCGTTACGGTGAG	110
		Anti-sense: TTTCCCGTTGAGCTGTTTGGCTGAAG
strH	SP0057	Sense: GTGTCAGCCCAAGCAGCTACCATACCAC	128
		Anti-sense: GGCCAAGGCTGGTACAATCTCGATCAGG
cbpI	SP0069	Sense: GCTATGAAGACAGGCTGGTACAAG	133
		Anti-sense: TCACAGCCAAAGCTCCTGAAC
nrdD	SP0202	Sense: TGCAACCAAGCGGATGTATCCAGACG	99
		Anti-sense: TGAAGGAAAGAACGGCAGCCCATAGG
	SP0287	Sense: CAGTCGGTGCCATTGCAGGTACTTCAAAC	103
		Anti-sense: GCTACAACCAAGGCTGTCAAACCAGTACG
caps4A	SP0346	Sense: GTCAGAGTATCCAGACTACGCATCGAAG	159
		Anti-sense: TCTGATCGCGACACCGAACTAATAGG
bgaA	SP0648^a	Sense: CAAGCCAGCCGTGAACGCTATAAGG	128
		Anti-sense: GAGTGGGCAGTCAGGGTGAATTTCC
gyrA	SP1219^a	Sense: GTGCTGCCGCTCAACGTTATACCGAGG	142
		Anti-sense: AAACGCGCTGGCAAGACCAAGGGTTCC
pyrR	SP1278	Sense: GACAGACCGCGAAGTTATCTTGGTGG	115
		Anti-sense: AACTGCTAAACTCACACGCGCAGGAC
	SP1679	Sense: GGACAGGGGATTACAGTTGATGAGATGG	149
		Anti-sense: GCAGTTGCAGCTACCCTACTTAAGATCG
	SP1680	Sense: GCCTGCATAACCATTTGGCTGATGTG	127
		Anti-sense: AGCATTCGACGAAGCGAGTGACATTG
	SP1688	Sense: AAGTGAACGAAGGGCTACTGCTACTGTC	136
		Anti-sense: GCTACCGATTGTAGCACCAGGTATTG
nanA	SP1693	Sense: GACATATTCGAAAGCGGGCGTAACGG	117
		Anti-sense: GCGTTCATCTGCACCTGCGATCAAAG
purR	SP1979^a	Sense: AGGCAGCCGTGTCTTGATTGTGG	120
		Anti-sense: TTGTCCGCAAAGACCGCTACACC

ISSN: 1756-0500