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Table 3 Common response genes to both A549 cells and THP-1 derived macrophages at 0.5 h and 1 h incubation time periods

From: Streptococcus pneumoniaeearly response genes to human lung epithelial cells

Function/gene name Protein TIGR4 genome acc. No. A549a THP-1b
    0.5 h 1 h 0.5 h 1 h
Cell envelope
cbpI c choline binding protein I SP0069   2.8   8.4
bgaA beta-galactosidase SP0648   17.0 3.4 26.9
nanA c neuraminidase A, authentic frameshift SP1693   16.5 3.9 47.1
Energy metabolism
agaS sugar isomerase domain protein AgaS SP0065   5.6   10.3
glgA glycogen synthase SP1124   3.8   5.4
  acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative SP1162   2.7 4.9 6.0
scrB sucrose-6-phosphate hydrolase SP1724 3.0 4.4 2.4 4.7
galT galactose-1-phosphate uridylyltransferase SP1852   2.7 2.6 4.4
galK galactokinase SP1853   2.3 2.3 2.8
recP transketolase SP2030   -3.6 -2.0 -2.5
gplK glycerol kinase SP2186   3.0   4.1
Hypothetical proteins       
  hypothetical protein SP0052 -3.5 -5.6 -2.6 -3.5
  hypothetical protein SP0067 2.4 2.1   4.1
  conserved hypothetical protein SP0159 -2.3   -2.0  
  conserved hypothetical protein SP0742   -2.9 -6.5 -3.2
  conserved hypothetical protein SP1003   2.1 2.1 3.4
  hypothetical protein SP1059   4.4 56.3 16.0
  conserved hypothetical protein SP1174   2.4 2.7 4.4
  hypothetical protein SP1198 2.7 2.6   2.8
  hypothetical protein SP1199 2.9 2.0   2.2
  hypothetical protein SP1677   10.3   14.6
  hypothetical protein SP1678 2.9 6.1   6.9
  hypothetical protein SP1679 4.6 9.6   6.6
  conserved hypothetical protein SP1680 5.3 11.5 2.0 14.6
Others
  lactose phosphotransferase system repressor, degenerate SP0169   2.2 15.4 6.0
acpP acyl carrier protein SP0418 -2.0   -2.3  
fabF 3-oxoacyl-(acyl-carrier-protein) synthase II SP0422 -2.4    -5.1
bta bacterocin transport accessory protein SP1499 -2.7 -2.4 -4.2 -2.2
Protein synthesis
rpsD ribosomal protein S4 SP0085   2.7   2.4
rpsJ ribosomal protein S10 SP0208   4.1   2.9
rpsC ribosomal protein S3 SP0215   2.0 2.2  
infC translation initiation factor IF-3 SP0959   2.5 2.2 2.3
rpmI ribosomal protein L35 SP0960   3.9 2.2  
rpsF ribosomal protein S6 SP1541 2.9 3.0   2.2
yfiA ribosomal subunit interface protein SP2206   -3.9 -2.6 -2.5
Purine and pyrimidine ribonucleotide biosynthesis
purC c phosphoribosylaminoimidazole-succinocarboxamide synthase SP0044 -5.1 -4.7 -2.4 -7.8
purH phosphoribosylaminoimidazolecarboxamide formyltransferase-IMP cyclohydrolase SP0050 -15.3 -4.1 -4.3 -5.5
purE c phosphoribosylaminoimidazole carboxylase, catalytic subunit SP0053 -6.4 -8.5   -4.2
carB carbamoyl-phosphate synthase, large subunit SP1275   -4.2   -2.6
pyrR pyrimidine operon regulatory protein SP1278 -2.1 -7.8 -2.3 -4.4
Transport
  PTS system, IIA component SP0064 2.2 3.4   6.5
  PTS system, mannose-specific IID component SP0282 -3.7   -2.4  
  xanthine-uracil permease family protein SP0287 -8.4 -5.3   -2.3
  PTS system, IIC component, putative SP0647   4.3 3.5 8.2
  ABC transporter, permease protein SP1688   5.3 2.4 13.6
  ABC transporter, permease protein SP1689   2.8 3.7 18.9
  ABC transporter, substrate-binding protein SP1690   2.1 3.9 21.6
msmE sugar ABC transporter, sugar-binding protein SP1897   2.1   4.1
malD maltodextrin ABC transporter, permease protein SP2110   2.6 2.1 6.4
Unknown function
  vanZ protein, putative SP0049 -2.9   -3.4 -4.9
  ACT domain protein SP0238 -2.1 -3.4   -2.3
  HIT family protein SP0521   -2.4   -2.1
  flavoprotein SP1231   -2.0 -2.3  
  1. a Genes identified in this study.
  2. b Genes identified in Song XM, Connor W, Hokamp K, Babiuk LA, Potter AA: Transcriptome studies on Streptococcus pneumoniae, illustration of early response genes to THP-1 human macrophages, submitted.
  3. c Genes that are also involved in pathogenesis according to TIGR genome annotation.