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Table 2 PCR reaction parameters

From: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

      Reaction parameters
Assay F & R [Primer] [Probe] Annealing temp 95°C Anneal 72°C Wavelength excite/acquire
Pj HSP70a 600 nM 200 nM 60°C 10 sec 10 sec 20 sec 530 nm/555 nm
mtLSU133 700 nM 100 nM 56°C 10 sec 20 sec 20 sec 530 nm/555 nm
CFP32 600 nM 75 nM 60°C 10 sec 20 sec 16 sec 530 nm/555 nm
IS1081 600 nM 75 nM 60°C 10 sec 20 sec 16 sec 530 nm/555 nm
16S MTb 600 nM 200 nM 60°C 10 sec 20 sec 20 sec 530 nm/555 nm
SPUD 600 nM 200 nM 56°C 10 sec 10 sec 20 sec 470 nm/510 nm