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Table 3 Primer and amplicon characteristics

From: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

  Primer Amplicon
Assay F/R Length bp Mw Tm°C %GC dG free energy 3' end stability dH enthalpy of the oligo Ds entropy of the oligo 5' end dG Size bp % GC
Pj HSP70a F 23 6949 59.43 43.48 -37.01 -8.51 -170.1 -0.45 -8.13 108 47.2
  R 20 6053 57.87 55 -34.14 -6.47 -153.4 -0.4 -7.58   
mtLSU133 F 20 6166 54.91 50 -32.16 -9.31 -146.8 -0.38 -8.03 133 32.2
  R 19 5777 54.46 52.63 -31.51 -8.53 -142.9 -0.37 -6.24   
CFP32 F 20 6233 57.32 50 -34.59 -8.57 -158.5 -0.42 -6.72 63 58.7
  R 18 5547 55.49 61.11 -30.83 -7.58 -134.5 -0.35 -9.6   
IS1081 F 22 6638 69.72 63.64 -41.97 -13.43 -174.5 -0.44 -8.29 135 67.4
  R 21 6496 67.86 61.9 -40.00 -8.48 -166.8 -0.43 -9.5   
16S MTb F 20 6175 56.81 50 -33.71 -7.58 -153.3 -0.4 -6.84 71 54.9
  R 20 6062 55.73 55 -32.04 -5.59 -143.4 -0.37 -8.27   
SPUD F 23 6999 62.07 43.48 -39.56 -8.2 -180.2 -0.47 -6.83 101 42.6
  R 23 6928 61.77 43.48 -37.62 -8.32 -167.4 -0.44 -6.71   
  1. N.B. Bold numbers correspond to factors potentially associated with susceptibility to inhibition.