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Figure 3 | BMC Research Notes

Figure 3

From: Quantification of circulating endothelial and progenitor cells: comparison of quantitative PCR and four-channel flow cytometry

Figure 3

Detection of progenitor cells spiked into peripheral blood samples. A) Four-channel flow cytometric analysis of cord blood derived progenitor cells spiked into PBMNC (n = 5). Progenitors were spiked at frequencies ranging from 0.001 to 1% into PBMNC of a healthy donor. (* p < 0.05 compared to unspiked controls). B) Quantitative PCR analysis of CEP marker gene expression in peripheral blood samples containing cord blood derived progenitor cells spiked at varying frequencies (0.001–1%; five independent experiments). Expression of CD34 and CD133 was analyzed in all samples relative to unspiked PBMNC. (* p < 0.05 compared to unspiked controls).

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