Gene | Sequence | Tm | Assay range cycles | Primer efficiency |
---|---|---|---|---|
EF1A (EEF1A1) | For: 5-cacacggctcacattgca Rev:5-cacgaacagcaaagcgacc | 86 | 12–30 | 98% |
CD34 (HPCA1) | For: 5-tccagagacaaccttgaagc Rev: 5-cttcttaaactccgcacagc | 85 | 18–32 | 100% |
CD133 (PROM1) | For: 5-ttgcggtaaaactggctaag Rev: 5-tgggcttgtcataacaggat | 81 | 20–36 | 100% |
KDR (VEGFR2) | For: 5-gtggggattgacttcaactg Rev: 5-tgtgctgttcttcttggtca | 85 | 17–35 | 98% |
CD144 (CDH5) | For: 5-ttcatgacgtgaacgacaac Rev: 5-tccaccacgatctcatacct | 89 | 17–35 | 84% |