Synthesis and transport of neutral lipids is hampered in dPPCS mutant egg chambers. Freshly dissected wt and dPPCS1/1 ovaries were stained with Nile red to visualize neutral lipids and dissected ovaries were directly analyzed by CLSM. Images represent single confocal scans. (A) Wt nurse cells produce high levels of neutral lipids, which are transported towards the oocyte, and are uniformly accumulated near the oocyte membrane. (B) dPPCS1/1 nurse cells produced less neutral lipids compared to wt nurse cells. No uniformly accumulated lipids were observed within the mutant oocyte compartment compared to wt. (oo) oocyte compartment. Scale bars: 100 μm.